EnzLab     Measurable Components

The procedures of enzymatic analysis (also called endpoint measurements or substrate analysis) are capable to quantify a number of components which are relevant to nutrients and clinical analysis. The components can be determined with high selectivity.

For many components ready-to-use test kits are commercially available. The table below lists components, for which test kits are available. See the information of the test-kit suppliers for more and up-to-date details. EnzLab includes predefined methods for all components listed.

Basic information and advice for further applications of enzymatic analysis is given by Bergmeyer.

Component	Working Range [g/L] *
Acetaldehyd	0,01 - 0,05
D-Malic Acid	0,06 - 0,3
L-Malic Acid	0,04 - 0,2
Formic Acid	0,02 - 0,1
Ammonia	0,01 - 0,08
L-Ascorbic Acid	0,03 - 0,2
Succinic Acid	0,05 - 0,4
Cholesterol	0,07 - 0,4
Citric Acid	0,04 - 0,4
Acetic Acid	0,03 - 0,15
Ethanol	0,01 - 0,06
D-Gluconic Acid, D-GIucono-d-lacton	0,1 - 0,6
D-Glucose	0,08 - 0,5
D-Glucose, D-Fructose	0,08 - 0,5 overall
Glycerol	0,04 - 0,4
L-Glutamic Acid	0,01 - 0,07
Urea, Ammonia	0,02 - 0,14/ 0,01 - 0,08
D-3-Hydroxybutyric Acid	0,04 - 0,12
D-Isocitric Acid	0,2 - 1,0
Lactose, D-Galactose	0,2 - 1,0 overall
Lactose, D-Glucose	0,1 - 1,0 overall
MaItose, Sucrose, D-Glucose	0,05 - 0,5 overall
D-Lactic Acid, L-Lactic Acid	0,03 - 0,2
L-Lactic Acid	0,03 - 0,2
Nitrate	0,03 - 0,3
Oxalic Acid	0,01 - 0,2
Raffinose	0,25 - 1,3
Sucrose, D-GIucose	0,05 - 0,8 overall
Sucrose, D-GIucose, D-Fructose	0,05 - 0,8 overall
D-Sorbit - Xylit	0,01 - 1,0
Starch	0,03 - 0,4
Sulfite	0,03 - 0,3
Xylit - D-Sorbit	0,01 - 1,0

Table taken from the r-biopharm information.

 * Working ranges refer to standard procedures according to kit instructions. With dilution or increased sample volume the working range can be extended. EnzLab methods can be adapted accordingly.